Ala-Glu-Gly-Asp-Asp-Pro-Ala-Lys-Ala-Ala-Phe-Asn-Se_H2N-Ala-Glu-Gly-Asp-Asp-Pro-Ala-Lys-Ala-Ala-Phe-Asn-Ser-Leu-Gln-Ala-Ser-Ala-Thr-Glu-Tyr-Ile-Gly-Tyr-Ala-Trp-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr-Ile-Gly-Ile-Lys-Leu-Phe-Lys-Lys-Phe-Thr-Ser-Ser-Lys-Ala-Ser-OH_H2N-AEGDDPAKAAFNSLQASATEYIGYAWAMVVVIVGATIGIKLFKKFTSSKAS-OH_未分类肽

多肽产品

Ala-Glu-Gly-Asp-Asp-Pro-Ala-Lys-Ala-Ala-Phe-Asn-Se

编号：202206

CAS号：

单字母：H2N-AEGDDPAKAAFNSLQASATEYIGYAWAMVVVIVGATIGIKLFKKFTSSKAS-OH

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编号:	202206
中文名称:	Ala-Glu-Gly-Asp-Asp-Pro-Ala-Lys-Ala-Ala-Phe-Asn-Se
单字母:	H2N-AEGDDPAKAAFNSLQASATEYIGYAWAMVVVIVGATIGIKLFKKFTSSKAS-OH
三字母:	H2N N端氨基：N-terminal amino group。在肽或多肽链中含有游离a-氨基的氨基酸一端。在表示氨基酸序列时，通常将N端放在肽链的左边。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Glu L-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -Gly 甘氨酸：glycine。系统命名为 2-氨基乙酸。是编码氨基酸中没有旋光性的最简单的氨基酸，因具有甜味而得名。符号：G，Gly。 -Asp L-天冬氨酸：aspartic acid。系统命名为(2S)-氨基-丁二酸。是编码氨基酸，又是神经递质。符号：D，Asp。D-天冬氨酸存在于多种细菌的细胞壁和短杆菌肽A中。 -Asp L-天冬氨酸：aspartic acid。系统命名为(2S)-氨基-丁二酸。是编码氨基酸，又是神经递质。符号：D，Asp。D-天冬氨酸存在于多种细菌的细胞壁和短杆菌肽A中。 -Pro L-脯氨酸：proline。系统命名为吡咯烷-(2S)-羧酸。为亚氨基酸。是编码氨基酸。在肽链中有特殊作用，如易形成顺式的肽键等。符号：P，Pro。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Lys L-赖氨酸：lysine。系统命名为(2S)-6-二氨基已酸。是编码氨基酸中的碱性氨基酸，哺乳动物的必需氨基酸。在蛋白质中的赖氨酸可以被修饰为多种形式的衍生物。符号：K，Lys。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Phe L-苯丙氨酸：phenylalanine。系统命名为(2S)-氨基-3-苯基丙酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：F，Phe。 -Asn 天冬酰胺：asparagine。L-天冬酰胺的系统命名为(2S)-氨基-3-氨酰基丙酸，是编码氨基酸。符号：N，Asn。D-天冬酰胺存在于短杆菌肽A分子中。 -Ser L-丝氨酸：serine。系统命名为(2S)-氨基-3-羟基丙酸。是编码氨基酸。因可从蚕丝中获得而得名。符号：S，Ser。在丝原蛋白及某些抗菌素中含有 D-丝氨酸。 -Leu L-亮氨酸：leucine。系统命名为(2S)-氨基-4-甲基戊酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：L，Leu。 -Gln L-谷氨酰胺：glutamine。系统命名为(2S)-氨基-4-氨酰基丁酸，是编码氨基酸。符号：GIn，Q。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Ser L-丝氨酸：serine。系统命名为(2S)-氨基-3-羟基丙酸。是编码氨基酸。因可从蚕丝中获得而得名。符号：S，Ser。在丝原蛋白及某些抗菌素中含有 D-丝氨酸。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Thr L-苏氨酸：threonine。系统命名为(2S)-氨基-(3R)-羟基丁酸。有两个手性中心，是编码氨基酸。是哺乳动物的必需氨基酸。符号：T，Thr。 -Glu L-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -Tyr L-酪氨酸：tyrosine。系统命名为(2S)-氨基-3-(4-羟基苯基)丙酸。是编码氨基酸。符号：Y，Tyr。 -Ile L-异亮氨酸：isoleucine。系统命名为(2S)-氨基-(3R)-甲基戊酸。是编码氨基酸。有两个手性碳原子，是哺乳动物的必需氨基酸。符号:I，Ile。 -Gly 甘氨酸：glycine。系统命名为 2-氨基乙酸。是编码氨基酸中没有旋光性的最简单的氨基酸，因具有甜味而得名。符号：G，Gly。 -Tyr L-酪氨酸：tyrosine。系统命名为(2S)-氨基-3-(4-羟基苯基)丙酸。是编码氨基酸。符号：Y，Tyr。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Trp L-色氨酸：tryptophan[e]。系统命名为(2S)-氨基-3-(3-吲哚基)丙酸。是编码氨基酸，哺乳动物的必需氨基酸。符号：W，Trp。某些抗菌素中含有 D-色氨酸。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Met L-甲硫氨酸：methionine又称“蛋氨酸”。系统命名为(2S)-氨基-4-甲硫基丁酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：M，Met。 -Val L-缬氨酸：valine。系统命名为(2S)-氨基-3-甲基丁酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：V，Val。在某些放线菌素如缬霉素中存在 D-缬氨酸。 -Val L-缬氨酸：valine。系统命名为(2S)-氨基-3-甲基丁酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：V，Val。在某些放线菌素如缬霉素中存在 D-缬氨酸。 -Val L-缬氨酸：valine。系统命名为(2S)-氨基-3-甲基丁酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：V，Val。在某些放线菌素如缬霉素中存在 D-缬氨酸。 -Ile L-异亮氨酸：isoleucine。系统命名为(2S)-氨基-(3R)-甲基戊酸。是编码氨基酸。有两个手性碳原子，是哺乳动物的必需氨基酸。符号:I，Ile。 -Val L-缬氨酸：valine。系统命名为(2S)-氨基-3-甲基丁酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：V，Val。在某些放线菌素如缬霉素中存在 D-缬氨酸。 -Gly 甘氨酸：glycine。系统命名为 2-氨基乙酸。是编码氨基酸中没有旋光性的最简单的氨基酸，因具有甜味而得名。符号：G，Gly。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Thr L-苏氨酸：threonine。系统命名为(2S)-氨基-(3R)-羟基丁酸。有两个手性中心，是编码氨基酸。是哺乳动物的必需氨基酸。符号：T，Thr。 -Ile L-异亮氨酸：isoleucine。系统命名为(2S)-氨基-(3R)-甲基戊酸。是编码氨基酸。有两个手性碳原子，是哺乳动物的必需氨基酸。符号:I，Ile。 -Gly 甘氨酸：glycine。系统命名为 2-氨基乙酸。是编码氨基酸中没有旋光性的最简单的氨基酸，因具有甜味而得名。符号：G，Gly。 -Ile L-异亮氨酸：isoleucine。系统命名为(2S)-氨基-(3R)-甲基戊酸。是编码氨基酸。有两个手性碳原子，是哺乳动物的必需氨基酸。符号:I，Ile。 -Lys L-赖氨酸：lysine。系统命名为(2S)-6-二氨基已酸。是编码氨基酸中的碱性氨基酸，哺乳动物的必需氨基酸。在蛋白质中的赖氨酸可以被修饰为多种形式的衍生物。符号：K，Lys。 -Leu L-亮氨酸：leucine。系统命名为(2S)-氨基-4-甲基戊酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：L，Leu。 -Phe L-苯丙氨酸：phenylalanine。系统命名为(2S)-氨基-3-苯基丙酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：F，Phe。 -Lys L-赖氨酸：lysine。系统命名为(2S)-6-二氨基已酸。是编码氨基酸中的碱性氨基酸，哺乳动物的必需氨基酸。在蛋白质中的赖氨酸可以被修饰为多种形式的衍生物。符号：K，Lys。 -Lys L-赖氨酸：lysine。系统命名为(2S)-6-二氨基已酸。是编码氨基酸中的碱性氨基酸，哺乳动物的必需氨基酸。在蛋白质中的赖氨酸可以被修饰为多种形式的衍生物。符号：K，Lys。 -Phe L-苯丙氨酸：phenylalanine。系统命名为(2S)-氨基-3-苯基丙酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：F，Phe。 -Thr L-苏氨酸：threonine。系统命名为(2S)-氨基-(3R)-羟基丁酸。有两个手性中心，是编码氨基酸。是哺乳动物的必需氨基酸。符号：T，Thr。 -Ser L-丝氨酸：serine。系统命名为(2S)-氨基-3-羟基丙酸。是编码氨基酸。因可从蚕丝中获得而得名。符号：S，Ser。在丝原蛋白及某些抗菌素中含有 D-丝氨酸。 -Ser L-丝氨酸：serine。系统命名为(2S)-氨基-3-羟基丙酸。是编码氨基酸。因可从蚕丝中获得而得名。符号：S，Ser。在丝原蛋白及某些抗菌素中含有 D-丝氨酸。 -Lys L-赖氨酸：lysine。系统命名为(2S)-6-二氨基已酸。是编码氨基酸中的碱性氨基酸，哺乳动物的必需氨基酸。在蛋白质中的赖氨酸可以被修饰为多种形式的衍生物。符号：K，Lys。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Ser L-丝氨酸：serine。系统命名为(2S)-氨基-3-羟基丙酸。是编码氨基酸。因可从蚕丝中获得而得名。符号：S，Ser。在丝原蛋白及某些抗菌素中含有 D-丝氨酸。 -OH C端羧基：C-terminal carboxyl group。在肽或多肽链中含有游离羧基的氨基酸一端。在表示氨基酸序列时，通常将C端放在肽链的右边。
氨基酸个数:	51
分子式:	C₂₄₄H₃₈₁N₅₉O₇₂S₁
平均分子量:	5325.05
精确分子量:	5321.77
等电点(PI):	10.48
pH=7.0时的净电荷数:	3.98
平均亲水性:	-0.28478260869565
疏水性值:	0.36
外观与性状:	粉末状固体
消光系数:	8480
来源:	人工化学合成，仅限科学研究使用，不得用于人体。
储存条件:	负80℃至负20℃
标签:	未分类肽

多肽H2N-Ala-Glu-Gly-Asp-Asp-Pro-Ala-Lys-Ala-Ala-Phe-Asn-Ser-Leu-Gln-Ala-Ser-Ala-Thr-Glu-Tyr-Ile-Gly-Tyr-Ala-Trp-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr-Ile-Gly-Ile-Lys-Leu-Phe-Lys-Lys-Phe-Thr-Ser-Ser-Lys-Ala-Ser-COOH的合成步骤：

1、合成CTC树脂：称取2.65g CTC Resin（如初始取代度约为1.15mmol/g）和3.66mmol Fmoc-Ser(tBu)-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入9.14mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Ser(tBu)-CTC Resin。结构图如下：

2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Ser(tBu)-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：

3、缩合：取9.14mmol Fmoc-Ala-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入18.29mmol DIPEA，8.69mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Ala-Ser(tBu)-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：

4、依次循环步骤二、步骤三，依次得到

H2N-Ala-Ser(tBu)-CTC Resin

Fmoc-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Asp(OtBu)-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Asp(OtBu)-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Gly-Asp(OtBu)-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Gly-Asp(OtBu)-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Glu(OtBu)-Gly-Asp(OtBu)-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

H2N-Glu(OtBu)-Gly-Asp(OtBu)-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

Fmoc-Ala-Glu(OtBu)-Gly-Asp(OtBu)-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin

以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。

最后再经过步骤二得到 H2N-Ala-Glu(OtBu)-Gly-Asp(OtBu)-Asp(OtBu)-Pro-Ala-Lys(Boc)-Ala-Ala-Phe-Asn(Trt)-Ser(tBu)-Leu-Gln(Trt)-Ala-Ser(tBu)-Ala-Thr(tBu)-Glu(OtBu)-Tyr(tBu)-Ile-Gly-Tyr(tBu)-Ala-Trp(Boc)-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr(tBu)-Ile-Gly-Ile-Lys(Boc)-Leu-Phe-Lys(Boc)-Lys(Boc)-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Ser(tBu)-CTC Resin，结构如下：

5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Ala-Glu-Gly-Asp-Asp-Pro-Ala-Lys-Ala-Ala-Phe-Asn-Ser-Leu-Gln-Ala-Ser-Ala-Thr-Glu-Tyr-Ile-Gly-Tyr-Ala-Trp-Ala-Met-Val-Val-Val-Ile-Val-Gly-Ala-Thr-Ile-Gly-Ile-Lys-Leu-Phe-Lys-Lys-Phe-Thr-Ser-Ser-Lys-Ala-Ser-COOH。结构图见产品结构图。

切割液选择：1）TFA：H2O=95%：5%

2）TFA：H2O：TIS=95%：2.5%：2.5%

3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%

（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）

6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度是否目标纯度。

7、最后总结：

杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。

以上所有内容，为专肽生物原创内容，请勿发布到其他网站上。
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