专肽生物_定制多肽合成服务_多肽合成定制服务_多肽合成厂家_多肽合成公司

一种高灵敏度钙蛋白酶荧光底物，吸收 / 发射 = 501/527 nm。通常基于罗丹明 110（Rh110）的蛋白酶底物比类似的 AMC 或 AFC 底物灵敏度更高。这种增强的灵敏度可能归因于酶促产物更强的荧光。无色无荧光的 (Z‑Ala‑Ala)₂Rh110 可水解为高荧光的罗丹明 110，其光谱特性优异，与大多数荧光仪器的最佳检测窗口匹配。另外，由于酶促产物（罗丹明 110）具有高摩尔消光系数（接近 100,000 cm⁻¹・mol⁻¹），(Z‑Ala‑Ala)₂Rh110 也可用于比色法检测钙蛋白酶。切割后最大吸收为 497 nm，最大发射为 527 nm。

A very sensitive fluorogenic calpain substrate, Abs/Em=501/527 nm. In general, Rh110-based protease substrates are much more sensitive than the analogous AMC or AFC-based substrate. This enhanced sensitivity might be attributed to the greater fluorescence of the enzymatic product. The colorless and nonfluorescent (Z-Ala-Ala)2Rh110 is hydrolyzed to the highly fluorescent Rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Alternatively, (Z-Ala-Ala)2Rh110 can also be used to detect calpain in a chromogenic mode since the enzymatic product (Rhodamine 110) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). After cleavage, Abs(max) is at 497 nm and Em(max) is at 527 nm.