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这种用于疟原虫血红蛋白酶 V（PMV）的 FRET 底物多肽源自富组蛋白 II（HRPII）的保守疟原虫输出元件（PEXEL）基序。PMV 是一种内质网天冬氨酸蛋白酶，可识别并切割恶性疟原虫输出蛋白 PEXEL 基序内的 RXL 序列，使其转运至宿主红细胞。

This FRET substrate peptide for Plasmepsin V (PMV) is derived from the conserved Plasmodium Export Element (PEXEL) motif of Histidine-Rich Protein II (HRPII). PMV is an ER aspartic protease that recognizes and cleaves the RXL sequence within the PEXEL motif of proteins exported by human malaria parasite Plasmodium falciparum, allowing them to translocate into host erythrocytes.

DABCYL标记说明

4-(4-二甲氨基苯基偶氮)苯甲酰基（Dabcyl）是一种发色团，常与荧光团（如EDANS）结合用作猝灭剂。Dabcyl/EDANS FRET（荧光共振能量转移）对的福斯特半径为3.3纳米。

4-(4-Dimethylaminophenylazo)benzoyl (Dabcyl) is a chromophor often used as a quencher in conjunction with a fluorophore (e.g., EDANS). Dabcyl/EDANS FRET pair have a Forster radius of 3.3 nm.

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Glossary: EDANS
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