专肽生物_定制多肽合成服务_多肽合成定制服务_多肽合成厂家_多肽合成公司

Secretoneurin, rat 是由分泌粒蛋白 II (SgII) 的蛋白水解过程产生的一种 33 个氨基酸多肽。Secretoneurin, rat 在体内和体外诱导大鼠纹状体多巴胺释放，对单核细胞和嗜酸性粒细胞有很强的趋化作用，而对粒细胞则没有。

Secretoneurin, rat, a 33-amino acid polypeptide, is generated by proteolytic processing of secretogranin II (SgII). Secretoneurin, rat induces dopamine release in the rat striatum in vivo and in vitro, and it exerts a very strong chemotactic effect on monocytes and eosinophils but not on granulocytes.

大鼠乙酸分泌蛋白是一种33个氨基酸的多肽，是一种通过分泌蛋白II（嗜铬粒蛋白C）的蛋白水解处理在大脑、肾上腺髓质和其他内分泌组织中产生的神经肽。内源性肽可以增强多巴胺的释放。

Secretoneurin, rat acetate, a 33-amino acid polypeptide, is a neuropeptide generated in brain, adrenal medulla and other endocrine tissues by proteolytic processing of secretogranin II (chromogranin C). The endogenous peptide can enhance dopamine release.

该肽是分泌素II的154至186个氨基酸片段，被称为分泌素尿蛋白。分泌素尿素是一种神经肽，通过分泌素II蛋白水解加工在脑、肾上腺髓质和其他内分泌组织中产生。分泌素作为直接的血管生成细胞因子，抑制内皮细胞（EC）凋亡，刺激EC增殖，并激活丝裂原活化蛋白激酶（MAPK）系统和Akt通路。

This peptide is amino acids 154 to 186 fragment of secretogranin II, designated secretoneurin. Secretoneurin is a neuropeptide generated in brain, adrenal medulla and other endocrine tissues by proteolytic processing of secretogranin II. Secretoneurin acts as direct angiogenic cytokine, inhibits endothelial cell (EC) apoptosis, stimulates EC proliferation, and activates the mitogen-activated protein kinase (MAPK) system and the Akt pathway.

多肽H2N-Thr-Asn-Glu-Ile-Val-Glu-Glu-Gln-Tyr-Thr-Pro-Gln-Ser-Leu-Ala-Thr-Leu-Glu-Ser-Val-Phe-Gln-Glu-Leu-Gly-Lys-Leu-Thr-Gly-Pro-Ser-Asn-Gln-COOH的合成步骤：

1、合成CTC树脂：称取0.82g CTC Resin（如初始取代度约为0.54mmol/g）和0.53mmol Fmoc-Gln(Trt)-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入1.33mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到  Fmoc-Gln(Trt)-CTC Resin。结构图如下：

2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Gln(Trt)-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：

3、缩合：取1.33mmol Fmoc-Asn(Trt)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入2.66mmol DIPEA，1.26mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Asn(Trt)-Gln(Trt)-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：

4、依次循环步骤二、步骤三，依次得到

H2N-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Ile-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Ile-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Glu(OtBu)-Ile-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Glu(OtBu)-Ile-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Asn(Trt)-Glu(OtBu)-Ile-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

H2N-Asn(Trt)-Glu(OtBu)-Ile-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

Fmoc-Thr(tBu)-Asn(Trt)-Glu(OtBu)-Ile-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin

以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。

最后再经过步骤二得到 H2N-Thr(tBu)-Asn(Trt)-Glu(OtBu)-Ile-Val-Glu(OtBu)-Glu(OtBu)-Gln(Trt)-Tyr(tBu)-Thr(tBu)-Pro-Gln(Trt)-Ser(tBu)-Leu-Ala-Thr(tBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Phe-Gln(Trt)-Glu(OtBu)-Leu-Gly-Lys(Boc)-Leu-Thr(tBu)-Gly-Pro-Ser(tBu)-Asn(Trt)-Gln(Trt)-CTC Resin，结构如下：

5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Thr-Asn-Glu-Ile-Val-Glu-Glu-Gln-Tyr-Thr-Pro-Gln-Ser-Leu-Ala-Thr-Leu-Glu-Ser-Val-Phe-Gln-Glu-Leu-Gly-Lys-Leu-Thr-Gly-Pro-Ser-Asn-Gln-COOH。结构图见产品结构图。

切割液选择：1）TFA：H2O=95%：5%

2）TFA：H2O：TIS=95%：2.5%：2.5%

3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%

（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）

6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。

7、最后总结：

杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。

以上所有内容，为专肽生物原创内容，请勿发布到其他网站上。