400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
Preptin是一种34个氨基酸的肽激素，可响应葡萄糖刺激与胰β细胞中的胰岛素共同分泌。
编号：190394
CAS号：315197-73-0
单字母：H2N-DVSTSQAVLPDDFPRYPVGKFFKFDTWRQSAGRL-OH
Preptin is a 34-amino acid peptide hormone, which is co-secreted with insulin from the pancreatic β-cells in response to glucose stimulation. It is osteogenic in vitro and in vivo and may act in concert with the other β-cell hormones insulin and amylin to stimulate bone formation in hyperinsulinemic states such as obesity.
Preptin (rat)是一个34个氨基酸的肽类激素。它从胰岛β细胞中为了应对葡萄糖的刺激与胰岛素被共分泌出来。在体外它具有成骨活性，在体内，在高胰岛素状态比如肥胖的情况下，它可能与其他β-细胞激素比如胰岛素和amylin一起刺激骨的形成。
| DOI | 名称 | |
|---|---|---|
| 10.1042/0264-6021:3600431 | Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion | 下载 |
| 10.1152/ajpendo.00642.2005 | Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo | 下载 |
多肽H2N-Asp-Val-Ser-Thr-Ser-Gln-Ala-Val-Leu-Pro-Asp-Asp-Phe-Pro-Arg-Tyr-Pro-Val-Gly-Lys-Phe-Phe-Lys-Phe-Asp-Thr-Trp-Arg-Gln-Ser-Ala-Gly-Arg-Leu-COOH的合成步骤：
1、合成CTC树脂：称取0.92g CTC Resin（如初始取代度约为0.99mmol/g）和1.09mmol Fmoc-Leu-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入2.73mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Leu-CTC Resin。结构图如下：
2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Leu-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取2.73mmol Fmoc-Arg(Pbf)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入5.46mmol DIPEA，2.6mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Arg(Pbf)-Leu-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Thr(tBu)-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Thr(tBu)-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ser(tBu)-Thr(tBu)-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Ser(tBu)-Thr(tBu)-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Val-Ser(tBu)-Thr(tBu)-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
H2N-Val-Ser(tBu)-Thr(tBu)-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
Fmoc-Asp(OtBu)-Val-Ser(tBu)-Thr(tBu)-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Asp(OtBu)-Val-Ser(tBu)-Thr(tBu)-Ser(tBu)-Gln(Trt)-Ala-Val-Leu-Pro-Asp(OtBu)-Asp(OtBu)-Phe-Pro-Arg(Pbf)-Tyr(tBu)-Pro-Val-Gly-Lys(Boc)-Phe-Phe-Lys(Boc)-Phe-Asp(OtBu)-Thr(tBu)-Trp(Boc)-Arg(Pbf)-Gln(Trt)-Ser(tBu)-Ala-Gly-Arg(Pbf)-Leu-CTC Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Asp-Val-Ser-Thr-Ser-Gln-Ala-Val-Leu-Pro-Asp-Asp-Phe-Pro-Arg-Tyr-Pro-Val-Gly-Lys-Phe-Phe-Lys-Phe-Asp-Thr-Trp-Arg-Gln-Ser-Ala-Gly-Arg-Leu-COOH。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
以上所有内容，为专肽生物原创内容，请勿发布到其他网站上。
