专肽生物_定制多肽合成服务_多肽合成定制服务_多肽合成厂家_多肽合成公司

一种HCV蛋白酶底物，在残基P1和P1之间结合酯键。由于剪切键与酰基酶中间体的酯交换反应很快，这种底物显示出非常高的kcat/Km值，能够检测亚纳米非结构蛋白3（NS3蛋白酶）浓度的活性。它被广泛用于NS3蛋白酶活性的连续测定。底物切割与酶浓度成正比，NS3的检测限在1 nM至250 pM之间。切割该底物后，可以在Abs/Em=355/500 nM处监测荧光。作为一种内部测序的荧光底物，它可用于监测HCV NS3蛋白酶活性。

A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.

该多肽是丙型肝炎病毒（HCV）蛋白酶底物，在 P1 与 P1' 残基之间包含酯键。由于易切割键可快速转酯化为酰基‑酶中间体，该底物表现出极高的 kcat/Km 值，可在纳摩尔以下非结构蛋白 3（NS3 蛋白酶）浓度下检测活性。它广泛用于 NS3 蛋白酶活性的连续检测。底物切割与酶浓度成正比，NS3 检测限在 1 nM 至 250 pM 之间。切割该底物后，可在吸收 / 发射 = 355/500 nm 下监测荧光。

This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.

DABCYL标记说明

4-(4-二甲氨基苯基偶氮)苯甲酰基（Dabcyl）是一种发色团，常与荧光团（如EDANS）结合用作猝灭剂。Dabcyl/EDANS FRET（荧光共振能量转移）对的福斯特半径为3.3纳米。

4-(4-Dimethylaminophenylazo)benzoyl (Dabcyl) is a chromophor often used as a quencher in conjunction with a fluorophore (e.g., EDANS). Dabcyl/EDANS FRET pair have a Forster radius of 3.3 nm.

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Glossary: EDANS
5-[(2-Aminoethyl)amino]naphthalene-1-sulfonyl (EDANS) is fluorophor with an excitation at 340 nm ▉ and emission of 490 nm ▉. EDANS is often paired together with acceptors like dabcyl for FRET experiements.
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EDANS标记说明

5-[(2-Aminoethyl)amino]naphthalene-1-sulfonyl (EDANS) is fluorophore with an excitation at 340 nm ▉ and emission of 490 nm ▉. EDANS are often paired together with acceptors like dabcyl for FRET experiments.