专肽生物_定制多肽合成服务_多肽合成定制服务_多肽合成厂家_多肽合成公司

ASV-30, a conformationally constrained analog of sauvagine, is a highly potent and selective antagonist directed against mouse CRF receptor type 2β (mCRFR2β). It has been shown to suppress agonist-induced adenylate cyclase activity in human embryonic kidney (HEK) 293 cells expressing mCRFR2β.

Antisauvagine-30是一种选择性、竞争性和强效的促肾上腺皮质激素释放因子CRF2受体拮抗剂，与大鼠CRF1和小鼠CRF2β受体结合的Kd值分别为153.6和1.4nM。它可以防止小鼠体内应激增强的恐惧条件和ERK1/2的MEK1/2依赖性激活。它抑制阴道刺激的cAMP在HEK-mCRF2β细胞中的积累。

Antisauvagine-30 is a selective, competitive and potent corticotropin-releasing factor CRF2 receptor antagonist with Kd values of 153.6 and 1.4 nM for binding to rat CRF1 and mouse CRF2β receptors respectively. It prevents stress-enhanced fear conditioning and MEK 1/2-dependent activation of ERK1/2 in mice in vivo. It inhibits sauvagine-stimulated cAMP accumulation in HEK-mCRF2β cells.

（D - 苯丙氨酸 ¹¹，组氨酸 ¹²）- 蛙皮降压肽（11-40）是一种神经肽蛙皮降压肽修饰片段，11 位为 D - 苯丙氨酸、12 位为组氨酸，增强受体相互作用及稳定性。蛙皮降压肽参与调控应激反应及水平衡。研究人员利用该类似物研究其对促肾上腺皮质激素释放因子（CRF）受体的作用及在下丘脑 - 垂体 - 肾上腺（HPA）轴中的作用。

(D-Phe11,His12)-Sauvagine (11-40) is a modified fragment of the neuropeptide sauvagine with D-phenylalanine at position 11 and histidine at position 12, enhancing receptor interaction and stability. Sauvagine is involved in regulating stress responses and water balance. Researchers use this analog to study its effects on corticotropin-releasing factor (CRF) receptors and its role in the hypothalamic-pituitary-adrenal (HPA) axis.

(D-Phe11,His12)-Sauvagine (11-40)，别名Antisauvagine-30，是一种蛙皮降压肽(sauvagine)构象约束类似物，它是一种有效的、竞争性的、选择性的2β型小鼠CRF受体(mCRFR2β)拮抗剂，针对小鼠 CRF2 细胞和大鼠 CRF1 受体的 Kd 值分别为 1.4 nM 和 153.6 nM。研究表明，在人胚胎期肾脏(HEK)293细胞表达mCRFR2β时，(D-Phe11,His12)-Sauvagine (11-40)具有抑制拮抗诱导的腺嘌呤核苷酸环化酶活性的功能。
 

很多蛋白在细胞中非常容易被降解，或被标记，进而被选择性地破坏。但含有部分D型氨基酸的多肽则显示了很强的抵抗蛋白酶降解能力。

多肽H2N-DPhe-His-Leu-Leu-Arg-Lys-Met-Ile-Glu-Ile-Glu-Lys-Gln-Glu-Lys-Glu-Lys-Gln-Gln-Ala-Ala-Asn-Asn-Arg-Leu-Leu-Leu-Asp-Thr-Ile-NH2的合成步骤：

1、合成MBHA树脂：取若干克的MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：

2、脱Fmoc：取1.72g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：

3、缩合：取1.55mmol Fmoc-Ile-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入3.1mmol DIPEA，1.47mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Ile-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：

4、依次循环步骤二、步骤三，依次得到

H2N-Ile-Linker-MBHA Resin

Fmoc-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Leu-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Leu-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-Leu-Leu-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-Leu-Leu-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-His(Trt)-Leu-Leu-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

H2N-His(Trt)-Leu-Leu-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

Fmoc-DPhe-His(Trt)-Leu-Leu-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin

以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。

最后再经过步骤二得到 H2N-DPhe-His(Trt)-Leu-Leu-Arg(Pbf)-Lys(Boc)-Met-Ile-Glu(OtBu)-Ile-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Glu(OtBu)-Lys(Boc)-Glu(OtBu)-Lys(Boc)-Gln(Trt)-Gln(Trt)-Ala-Ala-Asn(Trt)-Asn(Trt)-Arg(Pbf)-Leu-Leu-Leu-Asp(OtBu)-Thr(tBu)-Ile-Linker-MBHA Resin，结构如下：

5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-DPhe-His-Leu-Leu-Arg-Lys-Met-Ile-Glu-Ile-Glu-Lys-Gln-Glu-Lys-Glu-Lys-Gln-Gln-Ala-Ala-Asn-Asn-Arg-Leu-Leu-Leu-Asp-Thr-Ile-NH2。结构图见产品结构图。

切割液选择：1）TFA：H2O=95%：5%

2）TFA：H2O：TIS=95%：2.5%：2.5%

3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%

（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）

6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。

7、最后总结：

杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。

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