400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
这种肽来源于加压素-神经垂体素2-copeptin前体，是多种疾病的预后标志物，如败血症、社区获得性肺炎、慢性阻塞性肺衰竭、心力衰竭和心肌梗死。
编号：134468
CAS号：78362-34-2
单字母：H2N-ASDRSNATQLDGPAGALLLRLVQLAGAPEPFEPAQPDAY-OH
| 参考文献（References）: | J.Struck et al., Peptides, 26, 2500 (2005) N.G.Morgenthaler et al., Clin. Chem., 52, 112 (2006) S.Q.Khan et al., Circulation, 115, 2103 (2007) G.Szinnai et al., J. Clin. Endocrinol. Metab., 92, 3973 (2007) M.Katan et al., Crit. Care, 12, 117 (2008) R.Seligman et al., Crit. Care, 12, R11 (2008) |
| 溶解度: | Insoluble in water. Dissolve in a small amount of DMSO or 60% acetonitrile in water with 0.1% TFA. Then dilute with water or any desired buffer. |
这种肽来源于加压素-神经垂体素2-copeptin前体，是多种疾病的预后标志物，如败血症、社区获得性肺炎、慢性阻塞性肺衰竭、心力衰竭和心肌梗死。
This peptide derived from the vasopressin-neurophysin 2-copeptin precursor, a prognostic marker in a variety of diseases, such as sepsis, community-acquired pneumonia, chronic obstructive pulmonary failure, heart failure and myocardial infarction.
Copeptin是一种存在于血液和尿液中的人类蛋白质。它作为赖氨酸特异性蛋白酶（如组织蛋白酶D、弹性蛋白酶和胰蛋白酶）的竞争性抑制剂发挥作用。Copeptin与这些酶的活性位点结合，阻止它们切割底物。Copeptin也是一种用于免疫组织化学的抗原，使用针对它的抗体来检测各种癌症。生物素化的copeptin可用于与涂有抗生物素蛋白或链霉抗生物素的板或抗体（如过氧化物酶偶联抗体）结合，用于酶联免疫吸附测定（ELISA）。Copeptin与免疫系统针对它产生的一抗结合，然后使用荧光二抗或过氧化物酶偶联抗体进行检测。
Copeptin is a human protein that is found in the blood and urine. It functions as a competitive inhibitor of lysine-specific proteases, such as cathepsin D, elastase, and trypsin. Copeptin binds to the active site of these enzymes and prevents them from cleaving their substrates. Copeptin is also an antigen used in immunohistochemistry with an antibody against it for use in detection of various cancers. The biotinylated form of copeptin can be used for binding to avidin or streptavidin coated plates or antibodies (e.g., peroxidase-conjugated antibody) for use in enzyme-linked immunosorbent assays (ELISA). Copeptin binds to primary antibodies raised against it by the immune system, which are then detected using fluorescent secondary antibodies or peroxidase-conjugated antibodies.
和肽素（人）三氟乙酸盐代表前激素原加工的 C 端片段，在生化流程中具有出色稳定性。其两亲性支持神经内分泌研究中的折叠与相互作用分析。该肽有助于阐明前体成熟、肽转运与序列依赖性稳定性。应用延伸至分泌研究与结构 - 功能检测。
Copeptin (human) trifluoroacetate salt represents the C-terminal segment of pre-pro-hormonal processing, offering excellent stability in biochemical workflows. Its amphipathic nature supports folding and interaction analyses within neuroendocrine research. The peptide helps elucidate precursor maturation, peptide trafficking, and sequence-dependent stability. Applications extend to secretion studies and structure-function assays.
和肽素（人）是前加压素原 C 端片段，用于研究肽成熟与分泌通路。其序列支持前激素加工基序定位。研究人员通过构象趋势分析理解折叠中间体。该肽有助于加压素生物合成的广泛分析。
Copeptin (human) is the C-terminal segment of pre-pro-vasopressin used to study peptide maturation and secretory pathways. Its sequence supports mapping of prohormone processing motifs. Researchers evaluate its conformational tendencies to understand folding intermediates. The peptide contributes to broader analyses of vasopressin biosynthesis.
| DOI | 名称 | |
|---|---|---|
| 10.1016/j.peptides.2005.04.019 | Copeptin, a stable peptide derived from the vasopressin precursor, is elevated in serum of sepsis patients | 下载 |
| 10.1373/clinchem.2005.060038 | Assay for the measurement of copeptin, a stable peptide derived from the precursor of vasopressin | 下载 |
| 10.1161/CIRCULATIONAHA.106.685503 | C-terminal provasopressin (copeptin) as a novel and prognostic marker in acute myocardial infarction: Leicester Acute Myocardial Infarction Peptide (LAMP) study | 下载 |
| 10.1210/jc.2007-0232 | Changes in plasma copeptin, the c-terminal portion of arginine vasopressin during water deprivation and excess in healthy subjects | 下载 |
| 10.1186/cc6780 | Copeptin, a novel prognostic biomarker in ventilator-associated pneumonia | 下载 |
| 10.1186/cc6799 | Copeptin: a new and promising diagnostic and prognostic marker | 下载 |
多肽H2N-Ala-Ser-Asp-Arg-Ser-Asn-Ala-Thr-Gln-Leu-Asp-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg-Leu-Val-Gln-Leu-Ala-Gly-Ala-Pro-Glu-Pro-Phe-Glu-Pro-Ala-Gln-Pro-Asp-Ala-Tyr-COOH的合成步骤：
1、合成CTC树脂：称取2.77g CTC Resin（如初始取代度约为1.06mmol/g）和3.52mmol Fmoc-Tyr(tBu)-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入8.81mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Tyr(tBu)-CTC Resin。结构图如下：
2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Tyr(tBu)-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取8.81mmol Fmoc-Ala-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入17.62mmol DIPEA，8.37mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Ala-Tyr(tBu)-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Ala-Tyr(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Arg(Pbf)-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Arg(Pbf)-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Asp(OtBu)-Arg(Pbf)-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Asp(OtBu)-Arg(Pbf)-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
H2N-Ser(tBu)-Asp(OtBu)-Arg(Pbf)-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
Fmoc-Ala-Ser(tBu)-Asp(OtBu)-Arg(Pbf)-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Ala-Ser(tBu)-Asp(OtBu)-Arg(Pbf)-Ser(tBu)-Asn(Trt)-Ala-Thr(tBu)-Gln(Trt)-Leu-Asp(OtBu)-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg(Pbf)-Leu-Val-Gln(Trt)-Leu-Ala-Gly-Ala-Pro-Glu(OtBu)-Pro-Phe-Glu(OtBu)-Pro-Ala-Gln(Trt)-Pro-Asp(OtBu)-Ala-Tyr(tBu)-CTC Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Ala-Ser-Asp-Arg-Ser-Asn-Ala-Thr-Gln-Leu-Asp-Gly-Pro-Ala-Gly-Ala-Leu-Leu-Leu-Arg-Leu-Val-Gln-Leu-Ala-Gly-Ala-Pro-Glu-Pro-Phe-Glu-Pro-Ala-Gln-Pro-Asp-Ala-Tyr-COOH。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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