100513-58-4/100915-92-2_[Tyr0]Corticotropin Releasing Factor, human, rat_H2N-Tyr-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-CONH2_H2N-YSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEII-CONH2_未分类肽

多肽产品

[Tyr0]Corticotropin Releasing Factor, human, rat

编号：110109

CAS号：100513-58-4/100915-92-2

单字母：H2N-YSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEII-CONH2

立即咨询

 纠错

基本属性产品描述文献链接合成路线相关产品

编号:	110109
中文名称:	[Tyr0]Corticotropin Releasing Factor, human, rat
英文名:	[Tyr0]Corticotropin Releasing Factor, human, rat
CAS号:	100513-58-4/100915-92-2
单字母:	H2N-YSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEII-CONH2
三字母:	H2N N端氨基：N-terminal amino group。在肽或多肽链中含有游离a-氨基的氨基酸一端。在表示氨基酸序列时，通常将N端放在肽链的左边。 -Tyr L-酪氨酸：tyrosine。系统命名为(2S)-氨基-3-(4-羟基苯基)丙酸。是编码氨基酸。符号：Y，Tyr。 -Ser L-丝氨酸：serine。系统命名为(2S)-氨基-3-羟基丙酸。是编码氨基酸。因可从蚕丝中获得而得名。符号：S，Ser。在丝原蛋白及某些抗菌素中含有 D-丝氨酸。 -Glu L-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -Glu L-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -Pro L-脯氨酸：proline。系统命名为吡咯烷-(2S)-羧酸。为亚氨基酸。是编码氨基酸。在肽链中有特殊作用，如易形成顺式的肽键等。符号：P，Pro。 -Pro L-脯氨酸：proline。系统命名为吡咯烷-(2S)-羧酸。为亚氨基酸。是编码氨基酸。在肽链中有特殊作用，如易形成顺式的肽键等。符号：P，Pro。 -Ile L-异亮氨酸：isoleucine。系统命名为(2S)-氨基-(3R)-甲基戊酸。是编码氨基酸。有两个手性碳原子，是哺乳动物的必需氨基酸。符号:I，Ile。 -Ser L-丝氨酸：serine。系统命名为(2S)-氨基-3-羟基丙酸。是编码氨基酸。因可从蚕丝中获得而得名。符号：S，Ser。在丝原蛋白及某些抗菌素中含有 D-丝氨酸。 -Leu L-亮氨酸：leucine。系统命名为(2S)-氨基-4-甲基戊酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：L，Leu。 -Asp L-天冬氨酸：aspartic acid。系统命名为(2S)-氨基-丁二酸。是编码氨基酸，又是神经递质。符号：D，Asp。D-天冬氨酸存在于多种细菌的细胞壁和短杆菌肽A中。 -Leu L-亮氨酸：leucine。系统命名为(2S)-氨基-4-甲基戊酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：L，Leu。 -Thr L-苏氨酸：threonine。系统命名为(2S)-氨基-(3R)-羟基丁酸。有两个手性中心，是编码氨基酸。是哺乳动物的必需氨基酸。符号：T，Thr。 -Phe L-苯丙氨酸：phenylalanine。系统命名为(2S)-氨基-3-苯基丙酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：F，Phe。 -His L-组氨酸：histidine。系统命名为(2S)-氨基-3-(4-咪唑基)丙酸。其侧链带有弱碱性的咪唑基，为编码氨基酸。是幼小哺乳动物的必需氨基酸。符号：H，His。 -Leu L-亮氨酸：leucine。系统命名为(2S)-氨基-4-甲基戊酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：L，Leu。 -Leu L-亮氨酸：leucine。系统命名为(2S)-氨基-4-甲基戊酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：L，Leu。 -Arg L-精氨酸：arginine。系统命名为(2S)-氨基-5-胍基戊酸。在生理条件下带正电荷，为编码氨基酸。是幼小哺乳动物的必需氨基酸。符号：R，Arg。 -Glu L-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -Val L-缬氨酸：valine。系统命名为(2S)-氨基-3-甲基丁酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：V，Val。在某些放线菌素如缬霉素中存在 D-缬氨酸。 -Leu L-亮氨酸：leucine。系统命名为(2S)-氨基-4-甲基戊酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：L，Leu。 -Glu L-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -Met L-甲硫氨酸：methionine又称“蛋氨酸”。系统命名为(2S)-氨基-4-甲硫基丁酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：M，Met。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Arg L-精氨酸：arginine。系统命名为(2S)-氨基-5-胍基戊酸。在生理条件下带正电荷，为编码氨基酸。是幼小哺乳动物的必需氨基酸。符号：R，Arg。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Glu L-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -Gln L-谷氨酰胺：glutamine。系统命名为(2S)-氨基-4-氨酰基丁酸，是编码氨基酸。符号：GIn，Q。 -Leu L-亮氨酸：leucine。系统命名为(2S)-氨基-4-甲基戊酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：L，Leu。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -Gln L-谷氨酰胺：glutamine。系统命名为(2S)-氨基-4-氨酰基丁酸，是编码氨基酸。符号：GIn，Q。 -Gln L-谷氨酰胺：glutamine。系统命名为(2S)-氨基-4-氨酰基丁酸，是编码氨基酸。符号：GIn，Q。 -Ala 丙氨酸：alanine。L-丙氨酸的系统命名为(2S)-氨基丙酸，是编码氨基酸，也叫L-α-丙氨酸。符号：A，Ala。D-丙氨酸存在于多种细菌细胞壁的糖肽中。β-丙氨酸是维生素泛酸和辅酶A的组分。 -His L-组氨酸：histidine。系统命名为(2S)-氨基-3-(4-咪唑基)丙酸。其侧链带有弱碱性的咪唑基，为编码氨基酸。是幼小哺乳动物的必需氨基酸。符号：H，His。 -Ser L-丝氨酸：serine。系统命名为(2S)-氨基-3-羟基丙酸。是编码氨基酸。因可从蚕丝中获得而得名。符号：S，Ser。在丝原蛋白及某些抗菌素中含有 D-丝氨酸。 -Asn 天冬酰胺：asparagine。L-天冬酰胺的系统命名为(2S)-氨基-3-氨酰基丙酸，是编码氨基酸。符号：N，Asn。D-天冬酰胺存在于短杆菌肽A分子中。 -Arg L-精氨酸：arginine。系统命名为(2S)-氨基-5-胍基戊酸。在生理条件下带正电荷，为编码氨基酸。是幼小哺乳动物的必需氨基酸。符号：R，Arg。 -Lys L-赖氨酸：lysine。系统命名为(2S)-6-二氨基已酸。是编码氨基酸中的碱性氨基酸，哺乳动物的必需氨基酸。在蛋白质中的赖氨酸可以被修饰为多种形式的衍生物。符号：K，Lys。 -Leu L-亮氨酸：leucine。系统命名为(2S)-氨基-4-甲基戊酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：L，Leu。 -Met L-甲硫氨酸：methionine又称“蛋氨酸”。系统命名为(2S)-氨基-4-甲硫基丁酸。是编码氨基酸。是哺乳动物的必需氨基酸。符号：M，Met。 -Glu L-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -Ile L-异亮氨酸：isoleucine。系统命名为(2S)-氨基-(3R)-甲基戊酸。是编码氨基酸。有两个手性碳原子，是哺乳动物的必需氨基酸。符号:I，Ile。 -Ile L-异亮氨酸：isoleucine。系统命名为(2S)-氨基-(3R)-甲基戊酸。是编码氨基酸。有两个手性碳原子，是哺乳动物的必需氨基酸。符号:I，Ile。 -CONH2 C端酰胺化
氨基酸个数:	42
分子式:	C₂₁₇H₃₅₃N₆₁O₆₅S₂
平均分子量:	4920.62
精确分子量:	4917.56
等电点(PI):	6.52
pH=7.0时的净电荷数:	-0.55
平均亲水性:	0.0825
疏水性值:	-0.27
外观与性状:	白色粉末状固体
消光系数:	1490
来源:	人工化学合成，仅限科学研究使用，不得用于人体。
纯度:	95%、98%
盐体系:	可选TFA、HAc、HCl或其它
生成周期:	2-3周
储存条件:	负80℃至负20℃
标签:	未分类肽

"Tyrosyl-CRF is a protein-based activator of the catecholamine receptor. It can be used as a research tool to study the function of the catecholamine receptor and ion channels, or as an inhibitor of these receptors. Tyrosyl-CRF binds to the alpha subunit of the catecholamine receptor. This binding causes conformational changes in the receptor that activate it when tyrosine residues are present in the cytoplasmic domain. Tyrosyl-CRF is also able to bind to other proteins such as peptides and antibodies, which may have therapeutic potential for use in diseases such as cancer, Alzheimers disease, and Parkinsons disease."

DOI	名称
10.1016/s0014-2999(00)00412-x	Increased corticotropin-releasing factor concentrations in the bed nucleus of the stria terminalis of anhedonic rats	下载
10.1016/s0006-8993(00)02759-1	Influence of corticotrophin releasing factor on neuronal cell death in vitro and in vivo	下载
10.1046/j.1471-4159.2002.00903.x	Reciprocal regulation of calcium dependent and calcium independent cyclic AMP hydrolysis by protein phosphorylation	下载
10.1046/j.1471-4159.2003.01544.x	Corticotropin-releasing hormone induces proliferation and TNF-alpha release in cultured rat microglia via MAP kinase signalling pathways	下载
10.1016/j.neuroscience.2004.11.040	The corticotropin-releasing factor (CRF)-system and monoaminergic afferents in the central amygdala: investigations in different mouse strains and comparison with the rat	下载
10.1016/j.chroma.2006.01.102	Measurement of neuropeptides in clinical samples using chip-based immunoaffinity capillary electrophoresis	下载
10.1096/fj.05-5315fje	Activation of corticotropin-releasing factor receptor-2 causes bronchorelaxation and inhibits pulmonary inflammation in mice	下载
10.1677/JOE-08-0011	Interferon-gamma inhibits cellular proliferation and ACTH production in corticotroph tumor cells through a novel janus kinases-signal transducer and activator of transcription 1/nuclear factor-kappa B inhibitory signaling pathway	下载
10.1096/fj.09-131391	Annexin A1 regulates hormone exocytosis through a mechanism involving actin reorganization	下载
10.1016/0165-0173(90)90012-d	Physiological and behavioral responses to corticotropin-releasing factor administration: is CRF a mediator of anxiety or stress responses?	下载
10.1089/adt.2009.0232	Comparing label-free biosensors for pharmacological screening with cell-based functional assays	下载
10.1152/ajpendo.00155.2011	Opposite effects of serum- and glucocorticoid-regulated kinase-1 and glucocorticoids on POMC transcription and ACTH release	下载
10.1016/j.psyneuen.2012.07.016	Examining the role of endogenous orexins in hypothalamus-pituitary-adrenal axis endocrine function using transient dual orexin receptor antagonism in the rat	下载
10.1523/JNEUROSCI.1445-13.2013	NMDA receptor activation and calpain contribute to disruption of dendritic spines by the stress neuropeptide CRH	下载
10.1113/jphysiol.2015.284471	Large conductance Ca²⁺-activated K⁺ (BK) channels promote secretagogue-induced transition from spiking to bursting in murine anterior pituitary corticotrophs	下载
10.1002/cne.23796	Sociality and the telencephalic distribution of corticotrophin-releasing factor, urocortin 3, and binding sites for CRF type 1 and type 2 receptors: A comparative study of eusocial naked mole-rats and solitary Cape mole-rats	下载
10.15252/embj.201488795	The stress response neuropeptide CRF increases amyloid-β production by regulating γ-secretase activity	下载
10.1111/jnc.13195	Short-term modern life-like stress exacerbates Aβ-pathology and synapse loss in 3xTg-AD mice	下载
10.1371/journal.pone.0147250	Functional Impact of Corticotropin-Releasing Factor Exposure on Tau Phosphorylation and Axon Transport	下载
10.1016/j.cmet.2016.04.017	CRFR1 in AgRP Neurons Modulates Sympathetic Nervous System Activity to Adapt to Cold Stress and Fasting	下载
10.1016/j.cell.2016.08.067	A Cortical Circuit for Sexually Dimorphic Oxytocin-Dependent Anxiety Behaviors	下载
10.1038/s41598-017-18401-y	Murine splenic B cells express corticotropin-releasing hormone receptor 2 that affect their viability during a stress response	下载
10.1016/j.bbrc.2004.06.128	A novel soluble protein factor with non-opioid dynorphin A-binding activity	下载
10.1111/j.1365-2982.2005.00644.x	Divergent role for CRF1 and CRF2 receptors in the modulation of visceral pain	下载
10.1113/jphysiol.2007.150078	Corticotropin-releasing factor increases mouse ventral tegmental area dopamine neuron firing through a protein kinase C-dependent enhancement of Ih	下载

多肽H2N-Tyr-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2的合成步骤：

1、合成MBHA树脂：取若干克的MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：

2、脱Fmoc：取1.08g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：

3、缩合：取0.97mmol Fmoc-Ile-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入1.94mmol DIPEA，0.92mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Ile-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：

4、依次循环步骤二、步骤三，依次得到

H2N-Ile-Linker-MBHA Resin

Fmoc-Ile-Ile-Linker-MBHA Resin

H2N-Ile-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Glu(OtBu)-Glu(OtBu)-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Glu(OtBu)-Glu(OtBu)-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Ser(tBu)-Glu(OtBu)-Glu(OtBu)-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

H2N-Ser(tBu)-Glu(OtBu)-Glu(OtBu)-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

Fmoc-Tyr(tBu)-Ser(tBu)-Glu(OtBu)-Glu(OtBu)-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin

以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。

最后再经过步骤二得到 H2N-Tyr(tBu)-Ser(tBu)-Glu(OtBu)-Glu(OtBu)-Pro-Pro-Ile-Ser(tBu)-Leu-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Val-Leu-Glu(OtBu)-Met-Ala-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Leu-Ala-Gln(Trt)-Gln(Trt)-Ala-His(Trt)-Ser(tBu)-Asn(Trt)-Arg(Pbf)-Lys(Boc)-Leu-Met-Glu(OtBu)-Ile-Ile-Linker-MBHA Resin，结构如下：

5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Tyr-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2。结构图见产品结构图。

切割液选择：1）TFA：H2O=95%：5%

2）TFA：H2O：TIS=95%：2.5%：2.5%

3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%

（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）

6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度是否目标纯度。

7、最后总结：

杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。

以上所有内容，为专肽生物原创内容，请勿发布到其他网站上。
暂时没有数据

专肽生物_定制多肽合成服务_多肽合成定制服务_多肽合成厂家_多肽合成公司

多肽产品

[Tyr0]Corticotropin Releasing Factor, human, rat

QQ客服

QQ客服

联系电话

热线电话

微信客服

打开微信扫一扫

在线留言

回到顶部