由 40 个氨基酸组成的神经多肽，为选择性的 CRF2 受体激动剂。
编号：112200
CAS号：176591-49-4
单字母：H2N-DNPSLSIDLTFHLLRTLLELARTQSQRERAEQNRIIFDSV-CONH2
Urocortin, human 是由 40 个氨基酸组成的神经多肽，为选择性的 CRF2 受体激动剂，对 hCRF1，rCRF2α 和 mCRF2β 的 Ki 值分别为 0.4，0.3 和 0.5 nM。
Urocortin, human, a 40-aa neuropeptide, acts as a selective agonist of endogenous CRF2 receptor, with Kis of 0.4, 0.3, and 0.5 nM for hCRF1, rCRF2α and mCRF2β, respectively.
| DOI | 名称 | |
|---|---|---|
| 10.1016/s0167-0115(00)00180-4 | The neurobiology of urocortin | 下载 |
| 10.1016/s1471-4892(01)00117-5 | Corticotropin-releasing factor receptors 1 and 2 in anxiety and depression | 下载 |
多肽H2N-Asp-Asn-Pro-Ser-Leu-Ser-Ile-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Thr-Leu-Leu-Glu-Leu-Ala-Arg-Thr-Gln-Ser-Gln-Arg-Glu-Arg-Ala-Glu-Gln-Asn-Arg-Ile-Ile-Phe-Asp-Ser-Val-NH2的合成步骤：
1、合成MBHA树脂：取若干克的MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：
2、脱Fmoc：取2.73g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取2.46mmol Fmoc-Val-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入4.91mmol DIPEA，2.33mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Val-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Val-Linker-MBHA Resin
Fmoc-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Ser(tBu)-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Ser(tBu)-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Pro-Ser(tBu)-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Pro-Ser(tBu)-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Asn(Trt)-Pro-Ser(tBu)-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
H2N-Asn(Trt)-Pro-Ser(tBu)-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Asn(Trt)-Pro-Ser(tBu)-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Asp(OtBu)-Asn(Trt)-Pro-Ser(tBu)-Leu-Ser(tBu)-Ile-Asp(OtBu)-Leu-Thr(tBu)-Phe-His(Trt)-Leu-Leu-Arg(Pbf)-Thr(tBu)-Leu-Leu-Glu(OtBu)-Leu-Ala-Arg(Pbf)-Thr(tBu)-Gln(Trt)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Glu(OtBu)-Arg(Pbf)-Ala-Glu(OtBu)-Gln(Trt)-Asn(Trt)-Arg(Pbf)-Ile-Ile-Phe-Asp(OtBu)-Ser(tBu)-Val-Linker-MBHA Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Asp-Asn-Pro-Ser-Leu-Ser-Ile-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Thr-Leu-Leu-Glu-Leu-Ala-Arg-Thr-Gln-Ser-Gln-Arg-Glu-Arg-Ala-Glu-Gln-Asn-Arg-Ile-Ile-Phe-Asp-Ser-Val-NH2。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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