400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
Stresscopin 是促肾上腺皮质激素释放激素受体 2 型 (CRHR2) 的特异性配体。转录物在大脑和大多数分析组织中表达。腹膜内注射应激蛋白可抑制麻醉大鼠的热诱导水肿形成。它还减少食物摄入量并对胃排空活动表现出抑制作用。这种 CRHR2 激动剂可能代表一种在压力后维持体内平衡的内源性配体。
编号：177360
CAS号：352020-03-2
单字母：H2N-TKFTLSLDVPTNIMNLLFNIAKAKNLRAQAAANAHLMAQI-CONH2
应激素是促肾上腺皮质激素释放激素受体2型（CRHR2）的特异性配体。转录本在大脑和大多数组织中表达。腹腔注射stresscopin抑制麻醉大鼠热致水肿的形成。它还可以减少食物摄入量，并对胃排空活动有抑制作用。这种CRHR2激动剂可能是一种内源性配体，在应激后维持体内平衡。
Stresscopin is a specific ligand for the corticotropin-releasing hormone receptor type 2 (CRHR2). Transcripts are expressed in brain and most tissues analyzed. Intraperitoneal injections of stresscopin suppresses heat-induced edema formation in anesthetized rats. It also decreases food intake and exhibits an inhibitory effect on gastric emptying activity. This CRHR2 agonist might represent an endogenous ligand for maintaining homeostasis after stress.
应激蛋白是一种多肽，属于激活剂家族。它是一种分子“开关”，可以用来控制离子通道和蛋白质相互作用的活动。Stresscopin从人体组织中纯化，作为冻干粉末提供。
Stresscopin is a peptide that belongs to the family of activators. It is a molecular "switch" that can be used to control the activity of ion channels and protein interactions. Stresscopin is purified from human tissue and is supplied as lyophilized powder.
人应激肽是一种促肾上腺皮质激素释放因子相关多肽，具有两亲性螺旋与保守受体接触基序。序列结构支持溶液中稳定螺旋束形成。折叠动力学决定其选择性结合促肾上腺皮质激素释放因子受体的能力。该多肽用于应激轴生化研究与促肾上腺皮质激素释放因子家族多肽结构‑功能研究。
Stresscopin, human, is a CRF-related peptide with amphipathic helices and conserved receptor-contact motifs. Sequence organization supports stable helical bundles in solution. Folding dynamics underlie its ability to engage CRF receptors selectively. The peptide is used in stress-axis biochemical research and structure–function studies within the CRF peptide family.
| DOI | 名称 | |
|---|---|---|
| 10.1111/j.1471-4159.2007.05052.x | Expression, binding, and signaling properties of CRF2(a) receptors endogenously expressed in human retinoblastoma Y79 cells: passage-dependent regulation of functional receptors | 下载 |
多肽H2N-Thr-Lys-Phe-Thr-Leu-Ser-Leu-Asp-Val-Pro-Thr-Asn-Ile-Met-Asn-Leu-Leu-Phe-Asn-Ile-Ala-Lys-Ala-Lys-Asn-Leu-Arg-Ala-Gln-Ala-Ala-Ala-Asn-Ala-His-Leu-Met-Ala-Gln-Ile-NH2的合成步骤：
1、合成MBHA树脂：取若干克的MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：
2、脱Fmoc：取1.05g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取0.95mmol Fmoc-Ile-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入1.89mmol DIPEA，0.9mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Ile-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Ile-Linker-MBHA Resin
Fmoc-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Thr(tBu)-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Thr(tBu)-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Phe-Thr(tBu)-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Phe-Thr(tBu)-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Lys(Boc)-Phe-Thr(tBu)-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
H2N-Lys(Boc)-Phe-Thr(tBu)-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
Fmoc-Thr(tBu)-Lys(Boc)-Phe-Thr(tBu)-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Thr(tBu)-Lys(Boc)-Phe-Thr(tBu)-Leu-Ser(tBu)-Leu-Asp(OtBu)-Val-Pro-Thr(tBu)-Asn(Trt)-Ile-Met-Asn(Trt)-Leu-Leu-Phe-Asn(Trt)-Ile-Ala-Lys(Boc)-Ala-Lys(Boc)-Asn(Trt)-Leu-Arg(Pbf)-Ala-Gln(Trt)-Ala-Ala-Ala-Asn(Trt)-Ala-His(Trt)-Leu-Met-Ala-Gln(Trt)-Ile-Linker-MBHA Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Thr-Lys-Phe-Thr-Leu-Ser-Leu-Asp-Val-Pro-Thr-Asn-Ile-Met-Asn-Leu-Leu-Phe-Asn-Ile-Ala-Lys-Ala-Lys-Asn-Leu-Arg-Ala-Gln-Ala-Ala-Ala-Asn-Ala-His-Leu-Met-Ala-Gln-Ile-NH2。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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