400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
一种有效的 pTH 敏感性腺苷酸环化酶活性抑制剂和 pTH 与纯化犬肾皮质膜的结合。
编号：147859
CAS号：64297-16-1
单字母：H2N-SEIQF-Nle-HNLGKHLSS-Nle-ERVEWLRKKLQDVHNY-CONH2
A potent inhibitor of pTH-sensitive adenylate cyclase activity and pTH-binding to purified canine renal cortical membranes.
(正亮氨酸 8,18, 酪氨酸 34)- 牛甲状旁腺激素 (3-34) 酰胺是一种截短甲状旁腺激素类似物，设计用于探索受体结合而无需全长激活。正亮氨酸与酪氨酸取代改变多肽拓扑与信号偏向。该类似物有助于阐明甲状旁腺激素片段相互作用的结构基础。其特性支持专注于钙调控内分泌机制的研究。
(Nle8.18,Tyr34)-pTH (3-34) amide (bovine) is a truncated parathyroid hormone analog designed to explore receptor engagement without full-length activation. Norleucine and tyrosine substitutions modify peptide topology and signaling bias. The analog helps clarify the structural basis of PTH fragment interactions. Its properties support studies focused on calcium-regulated endocrine mechanisms.
| DOI | 名称 | |
|---|---|---|
| 10.1016/s0014-2999(03)01673-x | PTH2 receptor-mediated inhibitory effect of parathyroid hormone and TIP39 on cell proliferation | 下载 |
| 10.1158/0008-5472.CAN-08-4687 | The neuroendocrine-derived peptide parathyroid hormone-related protein promotes prostate cancer cell growth by stabilizing the androgen receptor | 下载 |
| 10.1073/pnas.87.18.6969 | Intrauterine occupancy controls expression of the parathyroid hormone-related peptide gene in preterm rat myometrium | 下载 |
| 10.1111/j.1476-5381.1982.tb09215.x | In vitro studies on an antagonist of parathyroid hormone [Nle-8, Nle-18, Tyr-34]bPTH-(3-34)amide | 下载 |
| 10.1073/pnas.80.5.1454 | Identification of adenylate cyclase-stimulating activity and cytochemical glucose-6-phosphate dehydrogenase-stimulating activity in extracts of tumors from patients with humoral hypercalcemia of malignancy | 下载 |
| 10.1210/endo.132.5.8386612 | Identical complementary deoxyribonucleic acids encode a human renal and bone parathyroid hormone (PTH)/PTH-related peptide receptor | 下载 |
多肽H2N-Ser-Glu-Ile-Gln-Phe-Nle-His-Asn-Leu-Gly-Lys-His-Leu-Ser-Ser-Nle-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Tyr-NH2的合成步骤：
1、合成MBHA树脂：取若干克的MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：
2、脱Fmoc：取2.44g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取2.2mmol Fmoc-Tyr(tBu)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入4.39mmol DIPEA，2.09mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Tyr(tBu)-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Gln(Trt)-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Gln(Trt)-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ile-Gln(Trt)-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ile-Gln(Trt)-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Ile-Gln(Trt)-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Ile-Gln(Trt)-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Glu(OtBu)-Ile-Gln(Trt)-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Ser(tBu)-Glu(OtBu)-Ile-Gln(Trt)-Phe-Nle-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Nle-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Ser-Glu-Ile-Gln-Phe-Nle-His-Asn-Leu-Gly-Lys-His-Leu-Ser-Ser-Nle-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Tyr-NH2。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
以上所有内容，为专肽生物原创内容，请勿发布到其他网站上。
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![64297-16-1,[Nle8, 18, Tyr34]-Parathyroid Hormone(3-34)amide,,H2N-Ser-Glu-Ile-Gln-Phe-Nle-His-Asn-Leu-Gly-Lys-His-Leu-Ser-Ser-Nle-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Tyr-NH2,H2N-SEIQF-Nle-HNLGKHLSS-Nle-ERVEWLRKKLQDVHNY-NH2,杭州专肽生物的产品](/static/ah/images/def.jpg)
