400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
编号：153729
CAS号：86292-93-5
单字母：H2N-FMHNLGKHLSSMERVEWLRKKLQDVHNY-CONH2
Tyr34-Parathyroid Hormone (Bovine, 7-34 amide) is a product sourced from bovine, is available as a 0.5mg vial and is related to the peptide hormone Parathyroid Hormone (PTH). During abnormal serum calcium levels PTH is secreted from the parathyroid gland, thus regulating calcium and phosphate levels in the body. PTH binds to its PTH receptor which is a G-protein coupled receptor that activated adenylate cyclase or phospholipase C to activate pathways involved in the mediation of bone resorption and bone formation.
酪氨酸 ³⁴ 甲状旁腺激素（7-34）酰胺（牛）是截短的 PTH 类似物，带有末端酪氨酸，能够标记并增强检测。该序列保留关键受体结合片段，同时缺乏激活起始的 N 端残基。其酰胺末端提高构象均匀性。研究人员用它进行 PTH 片段的结构和功能作图。
Tyr34 Parathyroid Hormone (7-34), amide, bovine is a truncated PTH analog with a terminal tyrosine that enables labeling and enhances detection. The sequence preserves key receptor-binding segments while lacking the activation-initiating N-terminal residues. Its amide terminus improves conformational uniformity. Researchers employ it in structural and functional mapping of PTH fragments.
(酪氨酸 34)- 牛甲状旁腺激素 (7-34) 酰胺是一种截短甲状旁腺激素（PTH）形式，34 位酪氨酸取代，提高受体结合亲和力与稳定性。该多肽片段对研究钙调控与骨代谢具有重要意义。研究人员用其探究甲状旁腺激素的受体结合动力学及其对骨转换与矿物质稳态的影响。
(Tyr34)-pTH (7-34) amide (bovine) is a truncated form of parathyroid hormone (PTH) with a tyrosine substitution at position 34, which enhances receptor-binding affinity and stability. This peptide fragment is important for studying calcium regulation and bone metabolism. Researchers use it to investigate the receptor-binding dynamics of PTH and its effects on bone turnover and mineral homeostasis.
| DOI | 名称 | |
|---|---|---|
| 10.1016/s0014-2999(03)01673-x | PTH2 receptor-mediated inhibitory effect of parathyroid hormone and TIP39 on cell proliferation | 下载 |
| 10.1126/science.6302844 | A parathyroid hormone inhibitor in vivo: design and biological evaluation of a hormone analog | 下载 |
多肽H2N-Phe-Met-His-Asn-Leu-Gly-Lys-His-Leu-Ser-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Tyr-NH2的合成步骤：
1、合成MBHA树脂：取若干克的MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：
2、脱Fmoc：取2.55g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取2.29mmol Fmoc-Tyr(tBu)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入4.59mmol DIPEA，2.18mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Tyr(tBu)-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Met-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
H2N-Met-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Phe-Met-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Phe-Met-His(Trt)-Asn(Trt)-Leu-Gly-Lys(Boc)-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Met-Glu(OtBu)-Arg(Pbf)-Val-Glu(OtBu)-Trp(Boc)-Leu-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Leu-Gln(Trt)-Asp(OtBu)-Val-His(Trt)-Asn(Trt)-Tyr(tBu)-Linker-MBHA Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Phe-Met-His-Asn-Leu-Gly-Lys-His-Leu-Ser-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Tyr-NH2。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
以上所有内容，为专肽生物原创内容，请勿发布到其他网站上。
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![86292-93-5,[Tyr34]-Parathyroid Hormone(7-34)amide, bovine,H2N-Phe-Met-His-Asn-Leu-Gly-Lys-His-Leu-Ser-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Tyr-NH2,H2N-FMHNLGKHLSSMERVEWLRKKLQDVHNY-NH2,杭州专肽生物的产品](/static/ah/images/def.jpg)
