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它对应于TAT蛋白的蛋白转导结构域，并与活化的半胱氨酸残基C（Npys）合成，其中Npys是3-硝基-2-吡啶磺酰基，用于活化半胱氨酸的S和在引入巯基时用于快速反应。它在N末端用C（Npys）合成，用于需要特定偶联反应的应用，并且具有用Abs/Em=494/518nm的FAM标记的荧光团。这种修饰已被用于使该肽成为可用于缀合研究的细胞穿透和载体肽。

It corresponds to the protein transduction domain of the TAT protein and is synthesized with an activated cysteine residue C(Npys), where Npys is 3-Nitro-2-pyridinesulfenyl group and is used for activating S of cysteine and for rapid reaction when a thiol group is introduced. It is synthesized with C(Npys) at the N-terminus for applications requiring specific conjugation reactions and has a fluorophore labeled with FAM having Abs/Em=494/518 nm. This kind of modification has been used to render this peptide as a cell penetrating and carrier peptide applicable in conjugation studies.

FAM标记说明:
Carboxyfluorescein (FAM) is fluorophor with an excitation at 492 nm ▉ and emission of 517 nm ▉. Donors like FAM and 5FAM are often paired together with acceptors (CPQ2) for FRET experiments.

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