400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
Tau Peptide是 Tau蛋白 的特定片段，通常由多个氨基酸组成，具有生物活性。这些肽段在神经退行性疾病（如 阿尔茨海默病 ）中与Tau蛋白的异常聚集和功能失调密切相关。
常见研究片段
294-305片段
由12个氨基酸组成（KDNIKHVPGGGS），可能干扰Tau蛋白的正常折叠和微管稳定性，参与疾病中的神经纤维缠结形成。
379-408片段
包含30个氨基酸（REN AKAKTDHGAEIVYKSPVVSGDTSP RHL），与Tau蛋白的磷酸化修饰及异常聚集机制相关，常用于病理机制研究。
307-321片段
由14个氨基酸组成（IVYKPVDLSKVETKI），可能通过改变Tau蛋白与其他分子的相互作用，影响细胞骨架稳定性和神经元功能。
潜在作用
这些肽段在正常生理状态下维持微管稳定，而疾病状态下可能因异常磷酸化或聚集导致神经退化。研究显示，特定肽段可通过阻断病理级联反应或减少神经纤维缠结，成为开发抗神经退行性疾病药物的新靶点。
| DOI | 名称 | |
|---|---|---|
| 10.1074/jbc.M113.494922 | Two novel Tau antibodies targeting the 396/404 region are primarily taken up by neurons and reduce Tau protein pathology | 下载 |
多肽H2N-Arg-Glu-Asn-Ala-Lys-Ala-Lys-Thr-Asp-His-Gly-Ala-Glu-Ile-Val-Tyr-Lys-Ser-Pro-Val-Val-Ser-Gly-Asp-Thr-Ser-Pro-Arg-His-Leu-COOH的合成步骤：
1、合成CTC树脂：称取0.36g CTC Resin（如初始取代度约为0.96mmol/g）和0.41mmol Fmoc-Leu-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入1.04mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Leu-CTC Resin。结构图如下：
2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Leu-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取1.04mmol Fmoc-His(Trt)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入2.07mmol DIPEA，0.98mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-His(Trt)-Leu-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-His(Trt)-Leu-CTC Resin
Fmoc-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Ala-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Ala-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Asn(Trt)-Ala-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Asn(Trt)-Ala-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Glu(OtBu)-Asn(Trt)-Ala-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
H2N-Glu(OtBu)-Asn(Trt)-Ala-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Glu(OtBu)-Asn(Trt)-Ala-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Arg(Pbf)-Glu(OtBu)-Asn(Trt)-Ala-Lys(Boc)-Ala-Lys(Boc)-Thr(tBu)-Asp(OtBu)-His(Trt)-Gly-Ala-Glu(OtBu)-Ile-Val-Tyr(tBu)-Lys(Boc)-Ser(tBu)-Pro-Val-Val-Ser(tBu)-Gly-Asp(OtBu)-Thr(tBu)-Ser(tBu)-Pro-Arg(Pbf)-His(Trt)-Leu-CTC Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Arg-Glu-Asn-Ala-Lys-Ala-Lys-Thr-Asp-His-Gly-Ala-Glu-Ile-Val-Tyr-Lys-Ser-Pro-Val-Val-Ser-Gly-Asp-Thr-Ser-Pro-Arg-His-Leu-COOH。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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