荧光底物 pERTKR-AMC 被弗林蛋白酶有效切割，弗林蛋白酶是酵母 Kex2 内切蛋白酶的哺乳动物同源物（kcat/Km 约 2·10⁴ M-¹s-¹）。作为与高尔基体膜相关的钙依赖性丝氨酸蛋白酶，弗林蛋白酶负责前体蛋白在配对碱性残基处的分泌加工。也被前蛋白转化酶 4 切割。Pyr-RTKR-AMC 是黄病毒素的极好底物。
编号：200338
CAS号：109481-23-4
单字母：Pyr-Q-OH
| 编号: | 200338 |
| 中文名称: | Pyr-Gln |
| CAS号: | 109481-23-4 |
| 单字母: | Pyr-Q-OH |
| 三字母: | Pyr 焦谷氨酸 -Gln谷氨酰胺 -OHC端羧基 |
| 氨基酸个数: | 1 |
| 分子式: | C10H15N3O5 |
| 平均分子量: | 257.24 |
| 精确分子量: | 257.1 |
| 等电点(PI): | - |
| pH=7.0时的净电荷数: | - |
| 平均亲水性: | 0.2 |
| 疏水性值: | -3.5 |
| 消光系数: | - |
| 来源: | 人工化学合成，仅限科学研究使用，不得用于人体。 |
| 储存条件: | 负80℃至负20℃ |
| 标签: | 谷氨酰胺类 |
The fluorogenic substrate pERTKR-AMC is efficiently cleaved by furin, a mammalian homolog of the yeast Kex2 endoprotease (kcat/Km approx. 2·10⁴ M-¹s-¹). As a calcium-dependent serine protease associated with Golgi membranes, furin is responsible for the secretory processing of precursor proteins at paired basic residues. Also cleaved by proprotein convertase 4. Pyr-RTKR-AMC is an excellent substrate for flavivirin.
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