甘丙肽样肽（GALP）是一种60氨基酸的神经肽，与甘丙肽不同，它具有非酰胺化的C端。
编号：146875
CAS号：245114-97-0
单字母：H2N-APAHRGRGGWTLNSAGYLLGPVLHLSSKANQGRKTDSALEILDLWKAIDGLPYSRSPRMT-OH
| 编号: | 146875 |
| 中文名称: | 甘丙肽样肽Galanin-Like Peptide (rat) |
| 英文名: | Galanin-Like Peptide (rat) |
| CAS号: | 245114-97-0 |
| 单字母: | H2N-APAHRGRGGWTLNSAGYLLGPVLHLSSKANQGRKTDSALEILDLWKAIDGLPYSRSPRMT-OH |
| 三字母: | H2N N端氨基 -Ala丙氨酸 -Pro脯氨酸 -Ala丙氨酸 -His组氨酸 -Arg精氨酸 -Gly甘氨酸 -Arg精氨酸 -Gly甘氨酸 -Gly甘氨酸 -Trp色氨酸 -Thr苏氨酸 -Leu亮氨酸 -Asn天冬酰胺 -Ser丝氨酸 -Ala丙氨酸 -Gly甘氨酸 -Tyr酪氨酸 -Leu亮氨酸 -Leu亮氨酸 -Gly甘氨酸 -Pro脯氨酸 -Val缬氨酸 -Leu亮氨酸 -His组氨酸 -Leu亮氨酸 -Ser丝氨酸 -Ser丝氨酸 -Lys赖氨酸 -Ala丙氨酸 -Asn天冬酰胺 -Gln谷氨酰胺 -Gly甘氨酸 -Arg精氨酸 -Lys赖氨酸 -Thr苏氨酸 -Asp天冬氨酸 -Ser丝氨酸 -Ala丙氨酸 -Leu亮氨酸 -Glu谷氨酸 -Ile异亮氨酸 -Leu亮氨酸 -Asp天冬氨酸 -Leu亮氨酸 -Trp色氨酸 -Lys赖氨酸 -Ala丙氨酸 -Ile异亮氨酸 -Asp天冬氨酸 -Gly甘氨酸 -Leu亮氨酸 -Pro脯氨酸 -Tyr酪氨酸 -Ser丝氨酸 -Arg精氨酸 -Ser丝氨酸 -Pro脯氨酸 -Arg精氨酸 -Met甲硫氨酸 -Thr苏氨酸 -OHC端羧基 |
| 氨基酸个数: | 60 |
| 分子式: | C288H461N87O83S1 |
| 平均分子量: | 6502.34 |
| 精确分子量: | 6498.42 |
| 等电点(PI): | 12.01 |
| pH=7.0时的净电荷数: | 7.45 |
| 平均亲水性: | -0.061224489795918 |
| 疏水性值: | -0.41 |
| 消光系数: | 13980 |
| 来源: | 人工化学合成，仅限科学研究使用，不得用于人体。 |
| 储存条件: | 负80℃至负20℃ |
| 标签: | 甘丙肽/甘丙样肽 |
Galanin-like peptide (GALP), first isolated from the porcine hypothalamus and subsequently also identified in rats and humans, is a 60 amino acid neuropeptide that unlike galanin, has a non-amidated C-terminus. Its amino acid residues (9-21) are identical to the biologically active N-terminal (1-13) fragment of galanin. In contrast to galanin which is relatively non-selective, receptor binding studies revealed that GALP had a high affinity for the galanin receptor 2 (GALR2) and a lower affinity for the GALR1 receptor. Furthermore, it could be demonstrated that GALP stimulates food intake with a ten fold higher orexigenic activity than galanin and may also affect emotional state in the CNS.
| DOI | 名称 | |
|---|---|---|
| 10.1016/s0304-3940(01)02515-0 | Galanin-like peptide stimulates food intake in the rat | 下载 |
| 10.1007/s12031-011-9546-y | Distribution of alarin immunoreactivity in the mouse brain | 下载 |
多肽H2N-Ala-Pro-Ala-His-Arg-Gly-Arg-Gly-Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-Val-Leu-His-Leu-Ser-Ser-Lys-Ala-Asn-Gln-Gly-Arg-Lys-Thr-Asp-Ser-Ala-Leu-Glu-Ile-Leu-Asp-Leu-Trp-Lys-Ala-Ile-Asp-Gly-Leu-Pro-Tyr-Ser-Arg-Ser-Pro-Arg-Met-Thr-COOH的合成步骤：
1、合成CTC树脂：称取1.16g CTC Resin（如初始取代度约为1.15mmol/g）和1.6mmol Fmoc-Thr(tBu)-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入4.0mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Thr(tBu)-CTC Resin。结构图如下：
2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Thr(tBu)-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取4.0mmol Fmoc-Met-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入8.0mmol DIPEA，3.8mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Met-Thr(tBu)-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Pro-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
H2N-Pro-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
Fmoc-Ala-Pro-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Ala-Pro-Ala-His(Trt)-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Gly-Trp(Boc)-Thr(tBu)-Leu-Asn(Trt)-Ser(tBu)-Ala-Gly-Tyr(tBu)-Leu-Leu-Gly-Pro-Val-Leu-His(Trt)-Leu-Ser(tBu)-Ser(tBu)-Lys(Boc)-Ala-Asn(Trt)-Gln(Trt)-Gly-Arg(Pbf)-Lys(Boc)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ile-Leu-Asp(OtBu)-Leu-Trp(Boc)-Lys(Boc)-Ala-Ile-Asp(OtBu)-Gly-Leu-Pro-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Ser(tBu)-Pro-Arg(Pbf)-Met-Thr(tBu)-CTC Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Ala-Pro-Ala-His-Arg-Gly-Arg-Gly-Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-Val-Leu-His-Leu-Ser-Ser-Lys-Ala-Asn-Gln-Gly-Arg-Lys-Thr-Asp-Ser-Ala-Leu-Glu-Ile-Leu-Asp-Leu-Trp-Lys-Ala-Ile-Asp-Gly-Leu-Pro-Tyr-Ser-Arg-Ser-Pro-Arg-Met-Thr-COOH。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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