400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
编号：136631
CAS号：
单字母：Biotinyl-Ahx-GSRAHSSHLKSKKGQSTSRHK-K(Ac)-LMFKTEGPDSD-CONH2
生物素可以与亲和素或者链霉亲和素有力结合，结合强度甚至接近共价键。生物素标记的肽通常用于免疫测定，组织细胞化学和基于荧光的流式细胞术。标记的抗生物素抗体也可以用来结合生物素化多肽。生物素标记常连接在赖氨酸侧链或者N末端。通常在多肽和生物素之间使用6-氨基己酸作为纽带，纽带能够灵活结合底物，并且在有空间位阻的情况下能结合地更好。专肽生物根据需求，向客户提供具有不同位点生物素标记多肽的定制合成。
专肽生物合成用于蛋白质-蛋白质相互作用研究的生物素化肽。尽管生物素可以在 N 端或 C 端引入（通过赖氨酸残基），但我们建议使用 N 端修饰，因为它成本低、成功率高、周转时间短且易于操作。因为多肽合成是从 C 端到 N 端合成的，因此，N 端修饰是 SPPS步骤的最后一步，不需要额外的特定缩合步骤。相比之下，C 端修饰需要额外的步骤，并且通常更复杂。当然，原则上生物素可以定位在任何地方。
生物素可以通过多种不同的接头或间隔物与肽分离。尽管如此，还是建议包含一个灵活的间隔物，例如 Ahx（一个 6 碳接头），以使生物素标签更加稳定或灵活。
专肽生物在 N 端或 C 端提供生物素化：生物素-N 端、赖氨酸-生物素-肽中间和赖氨酸-生物素-C 端。
专肽生物还可以使用 Ahx 接头或长碳 (LC) 接头提供生物素化：生物素-Ahx-N 末端、Lys-Ahx-生物素-肽中间、Lys-Ahx-生物素-C-末端。
（生物素结构）
示例：
GRGDS在N端和C端标记生物素的结构展示。
1、GRGDS在N端标记生物素，不增加Ahx 接头
2、GRGDS在N端标记生物素，增加一个Ahx 接头
3、GRGDS在C端标记生物素，不增加Ahx 接头
4、GRGDS在C端标记生物素，增加一个Ahx 接头。
多肽Biotin-Ahx-Gly-Ser-Arg-Ala-His-Ser-Ser-His-Leu-Lys-Ser-Lys-Lys-Gly-Gln-Ser-Thr-Ser-Arg-His-Lys-Lys(Ac)-Leu-Met-Phe-Lys-Thr-Glu-Gly-Pro-Asp-Ser-Asp-NH2的合成步骤：
1、合成MBHA树脂：取若干克MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：
2、脱Fmoc：取2.79g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取2.51mmol Fmoc-Asp(OtBu)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入5.02mmol DIPEA，2.39mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Asp(OtBu)-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Arg(Pbf)-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Arg(Pbf)-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Gly-Ser(tBu)-Arg(Pbf)-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
H2N-Gly-Ser(tBu)-Arg(Pbf)-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
Fmoc-Ahx-Gly-Ser(tBu)-Arg(Pbf)-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Ahx-Gly-Ser(tBu)-Arg(Pbf)-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHA Resin，结构如下：
5、生物素反应连接：在上述树脂中，加入适当DMF后，再加入2.51mmol 生物素到树脂中，再加入5.02mmol DIPEA、2.39mmol HBTU，鼓氮气反应30分钟。用2倍树脂体积的DMF 洗涤3次树脂（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。 得到Biotin-Ahx-Gly-Ser(tBu)-Arg(Pbf)-Ala-His(Trt)-Ser(tBu)-Ser(tBu)-His(Trt)-Leu-Lys(Boc)-Ser(tBu)-Lys(Boc)-Lys(Boc)-Gly-Gln(Trt)-Ser(tBu)-Thr(tBu)-Ser(tBu)-Arg(Pbf)-His(Trt)-Lys(Boc)-Lys(Ac)-Leu-Met-Phe-Lys(Boc)-Thr(tBu)-Glu(OtBu)-Gly-Pro-Asp(OtBu)-Ser(tBu)-Asp(OtBu)-Linker-MBHAResin。 结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品Biotin-Ahx-Gly-Ser-Arg-Ala-His-Ser-Ser-His-Leu-Lys-Ser-Lys-Lys-Gly-Gln-Ser-Thr-Ser-Arg-His-Lys-Lys(Ac)-Leu-Met-Phe-Lys-Thr-Glu-Gly-Pro-Asp-Ser-Asp-NH2。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
以上所有内容，为专肽生物原创内容，请勿发布到其他网站上。
![[Lys(Ac)382]-p53(361-393), biotinlabeled,Biotin-Ahx-Gly-Ser-Arg-Ala-His-Ser-Ser-His-Leu-Lys-Ser-Lys-Lys-Gly-Gln-Ser-Thr-Ser-Arg-His-Lys-Lys(Ac)-Leu-Met-Phe-Lys-Thr-Glu-Gly-Pro-Asp-Ser-Asp-NH2,Biotin-Acp-GSRAHSSHLKSKKGQSTSRHK-K(Ac)-LMFKTEGPDSD-NH2,杭州专肽生物的产品](/static/ah/images/def.jpg)
