400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
一种在下丘脑视交叉上核（SCN）中特异性表达的神经介素U相关神经肽。RNM通过自分泌和/或旁分泌作用参与昼夜节律的调节。
编号：119765
CAS号：843782-19-4
单字母：H2N-LPRLLHTDSRMATIDFPKKDPTTSLGRPFFLFRPRN-CONH2
| 参考文献（References）: | T.Ida et al., Endocrinology, 146, 4217 (2005) K.Mori et al., EMBO J., 24, 325 (2005) |
一种在下丘脑视交叉上核（SCN）中特异性表达的神经介素U相关神经肽。RNM通过自分泌和/或旁分泌作用参与昼夜节律的调节。RNM显示出有效的厌食作用，并抵消了神经肽Y，生长素释放肽和刺豚鼠相关蛋白诱导的食物摄入。
A neuromedin U-related neuropeptide specifically expressed in the suprachiasmatic nuclei (SCN) of the hypothalamus. rNMS is implicated in the regulation of circadian rhythms through autocrine and/or paracrine actions. rNMS showed potent anorexigenic effects and counteracted neuropeptide Y, ghrelin and agouti-related protein-induced food intake.
Neuromedin S（大鼠）是一种肽，是蛋白质相互作用的有效抑制剂。它与受体的细胞外结构域结合并阻断其与配体的相互作用。这可以用作研究蛋白质在细胞生物学，药理学或离子通道中的作用的研究工具。Neuromedin S（大鼠）是一种高纯度肽，CAS号为843782-19-4，已显示其在体外和体内抑制配体与受体的结合。Neuromedin S（大鼠）还在微摩尔浓度下抑制激动剂诱导的离子通道激活，并在纳摩尔浓度下阻断电压门控钠电流。它已被用于通过阻断神经毒素与神经胶蛋白的相互作用来研究神经毒素在神经元之间突触传递中的作用。
Neuromedin S (Rat) is a peptide that is a potent inhibitor of protein interactions. It binds to the extracellular domain of the receptor and blocks its interaction with the ligand. This can be used as a research tool for studying the role of proteins in cell biology, pharmacology, or ion channels. Neuromedin S (Rat) is a high-purity peptide with CAS No. 843782-19-4 that has been shown to inhibit ligand binding to receptors in vitro and in vivo. Neuromedin S (Rat) also inhibits agonist-induced activation of ion channels at micromolar concentrations and blocks voltage-gated sodium currents at nanomolar concentrations. It has been used to study the role of neurexins in transmission at synapses between neurons by blocking their interaction with neuroligins.
神经介素 S（大鼠）是结构上与神经介素 U 相关的内源性配体，参与昼夜节律和应激相关通路。其序列支持富含螺旋的构象和受体结合的分析。研究人员用它比较与神经介素 U 的功能重叠。该肽有助于大鼠神经内分泌信号传导回路的作图。
Neuromedin S (rat) is an endogenous ligand structurally related to neuromedin U, participating in circadian and stress-related pathways. Its sequence supports analyses of helix-rich conformations and receptor binding. Researchers employ it to compare functional overlap with neuromedin U. The peptide aids mapping of rat neuroendocrine signaling circuits.
多肽H2N-Leu-Pro-Arg-Leu-Leu-His-Thr-Asp-Ser-Arg-Met-Ala-Thr-Ile-Asp-Phe-Pro-Lys-Lys-Asp-Pro-Thr-Thr-Ser-Leu-Gly-Arg-Pro-Phe-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2的合成步骤：
1、合成MBHA树脂：取若干克的MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：
2、脱Fmoc：取2.07g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取1.86mmol Fmoc-Asn(Trt)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入3.73mmol DIPEA，1.77mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Asn(Trt)-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Asn(Trt)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Leu-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Leu-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Leu-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Arg(Pbf)-Leu-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Pro-Arg(Pbf)-Leu-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
H2N-Pro-Arg(Pbf)-Leu-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
Fmoc-Leu-Pro-Arg(Pbf)-Leu-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Leu-Pro-Arg(Pbf)-Leu-Leu-His(Trt)-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Arg(Pbf)-Met-Ala-Thr(tBu)-Ile-Asp(OtBu)-Phe-Pro-Lys(Boc)-Lys(Boc)-Asp(OtBu)-Pro-Thr(tBu)-Thr(tBu)-Ser(tBu)-Leu-Gly-Arg(Pbf)-Pro-Phe-Phe-Leu-Phe-Arg(Pbf)-Pro-Arg(Pbf)-Asn(Trt)-Linker-MBHA Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Leu-Pro-Arg-Leu-Leu-His-Thr-Asp-Ser-Arg-Met-Ala-Thr-Ile-Asp-Phe-Pro-Lys-Lys-Asp-Pro-Thr-Thr-Ser-Leu-Gly-Arg-Pro-Phe-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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