2014.12.01，2014.12.01，Professor Sun Baolin Published a paper entitled:“Mechanism of Reduced Vancomycin Susceptibility Conferred by walKMutation in Community-Acquired Methicillin-Resistant Staphylococcus aureus Strain MW2” in Antimicrobial Agents and Chemotherapy，online
Author：Hu Jinfeng、Zhang Xu、Liu Xiaoyu、Chen Chuan、Sun Baolin
Abstract: Point mutations are often associated with vancomycin resistance in Staphylococcus aureus with unclear molecular mechanism. Here we observed that walK (G223D) mutation caused decreased expression of genes associated with cell wall metabolism, decreased autolytic activity, thickened cell wall, and reduced vancomycin susceptibility. Phosphorylation assay showed that WalK (G223D) exhibited reduced autophosphorylation, which led to reduced phosphorylation of WalR. Electrophoretic mobility shift assay indicated that WalK (G223D)-phosphorylated WalR had a reduced binding capacity to atlA promoter.
DOI information: 10.1128/AAC.04290-14
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