Preparation and characterization of Lecithin-heparin intercalated in montmorillonite nanocomposite

Meng, N (Meng, Na)[ 1,3 ] ; Chu, XH (Chu, Xiaohong)[ 1,2,4 ] ; Ge, MQ (Ge, Man Qing)[ 1,3 ] ; Zhang, M (Zhang, Ming)[ 1,2,4 ] ; Sun, BH (Sun, Baohong)[ 1,2,4 ] ; Su, YT (Su, Yu-Tian)[ 1,2,4 ] ; Zhou, NL (Zhou, Ning-Lin)[ 1,2,4 ]*（周宁琳）

[ 1 ] Nanjing Normal Univ, Jiangsu Engn Res Ctr Biomed Funct Mat, Nanjing 210046, Jiangsu, Peoples R China

[ 2 ] Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Key Lab Biofunct Mat, Nanjing 210097, Jiangsu, Peoples R China

[ 3 ] Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Supramol Med Mat & Applicat, Nanjing 210046, Jiangsu, Peoples R China

[ 4 ] Nanjing Univ, Jiangsu Technol Res Ctr Interfacial Chem & Chem E, Nanjing 210093, Jiangsu, Peoples R China

APPLIED CLAY SCIENCE，201809,162,454-460

In this work, a study of Lecithin-heparin intercalated in montmorillonite was performed in order to synthesize an antithrombogenic hybrid. The hybrid of montmorillonite (Mt)/Lecithin-heparin (LEC-HEP) was synthesized by the intercalation method under mechanical stirring. The composite was characterized by X-ray diffraction (XRD) analysis, X-ray fluorescence (XRF) and C, H, and N elemental analyses, zeta potentials, Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) techniques. The d(001)-value was increased by LEC-HEP intercalation. Fourier transform infrared spectroscopy also confirmed the intercalation of Lecithin-heparin into Mt interlayer space. In vitro release study of the antithrombogenic drug-Mt intercalates in phosphate buffer saline (pH 7.4) media at 37 degrees C was investigated. The heparin showed an initial burst effect for 24 h and then continuously released for 30 d. In-vitro blood compatibility of the Mt/LEC-HEP was carried out via hemolysis assay. The Mt/LEC-HEP exhibited hemolysis below 5% which is permissible for biomaterials. These modified materials have the potential for being used as blood contact materials.

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https://www.sciencedirect.com/science/article/pii/S0169131718302734?via%3Dihub